Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena

  title={Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena},
  author={K E Kruger and Paula J. Grabowski and Arthur J. Zaug and Julie Sands and Daniel E. Gottschling and Thomas R. Cech},

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R2 Retrotransposons Encode a Self-Cleaving Ribozyme for Processing from an rRNA Cotranscript

It is shown here that processing of the rRNA cotranscript at the 5′ end of the R2 element in Drosophila simulans is rapid and utilizes an unexpected mechanism, capable of rapid and efficient self-cleavage of the 28S-R2 cOTranscript.

Self-splicing RNA: implications for evolution.

  • T. Cech
  • Biology
    International review of cytology
  • 1985

Catalysis of RNA cleavage by a ribozyme derived from the group I intron of Anabaena pre-tRNA(Leu).

Two group I ribozymes, although differing in the identity of many of their active site nucleotides, nevertheless provide functionally similar active sites for sequence-specific RNA cleavage, and are concluded to be limited by the actual chemical cleavage step.

Deletion of nonconserved helices near the 3' end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity.

Analysis of the cryptic site suggests that choice of the 3' splice site may not only depend on sequence but also on proximity to P9, a conserved helix at the 5' edge of the deletion point, which may be responsible for the reduced activity and accuracy of reactions with mutant precursors.



In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.

It is demonstrated that the enzyme activities necessary for the endonucleolytic cleavage as well as for the ligation of the transcript are associated with the isolated macronuclear rRNA genes of Tetrahymena thermophila.

Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.

  • T. CechD. Rio
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1979
R-loop hybridization and electron microscopy were used to map the RNA transcription products of the extrachromosomal rRNA genes of Tetrahymena thermophila, and preliminary evidence for a primary transcript that contains the intervening sequence was found.

The intervening sequence excised from the ribosomal RNA precursor of Tetrahymena contains a 5-terminal guanosine residue not encoded by the DNA.

The splicing mechanisms are fundamentally different for yeast tRNA intervening sequences and Tetrahymena thermophila transcripts, leading to the conclusion that the splicing mechanism for these two classes of transcripts is fundamentally different.

The nucleotide sequence at the transcription termination site of the ribosomal RNA gene in Tetrahymena thermophila.

The sequence of 415 nucleotides surrounding the transcription termination site for ribosomal RNA in Tetrahymena thermophila has been determined and a cluster of T's at the putative termination site, and several other T clusters are found further downstream.

Sequence of a ribosomal RNA gene intron from Tetrahymena

The sequence of a ribosomal RNA gene intron from Tetrahymena is reported in which intron–exon junctions differ from those analysed to date, suggesting different splicing activities are necessary for the processing of introns in mRN A and tRN A precursors.