Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena
@article{Kruger1982SelfsplicingRA, title={Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena}, author={K E Kruger and Paula J. Grabowski and Arthur J. Zaug and Julie Sands and Daniel E. Gottschling and Thomas R. Cech}, journal={Cell}, year={1982}, volume={31}, pages={147-157} }
1,962 Citations
5' exon requirement for self-splicing of the Tetrahymena thermophila pre-ribosomal RNA and identification of a cryptic 5' splice site in the 3' exon.
- BiologyJournal of molecular biology
- 1987
New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicing.
- Biology, ChemistryJournal of molecular biology
- 1986
Origin of the phosphate at the ligation junction produced by self-splicing of Tetrahymena thermophila pre-ribosomal RNA.
- Biology, ChemistryJournal of molecular biology
- 1987
Intermolecular exon ligation of the rRNA precursor of tetrahymena: Oligonucleotides can function as 5′ exons
- Biology, ChemistryCell
- 1985
R2 Retrotransposons Encode a Self-Cleaving Ribozyme for Processing from an rRNA Cotranscript
- BiologyMolecular and Cellular Biology
- 2010
It is shown here that processing of the rRNA cotranscript at the 5′ end of the R2 element in Drosophila simulans is rapid and utilizes an unexpected mechanism, capable of rapid and efficient self-cleavage of the 28S-R2 cOTranscript.
Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript
- BiologyCell
- 1986
Catalysis of RNA cleavage by a ribozyme derived from the group I intron of Anabaena pre-tRNA(Leu).
- Biology, ChemistryBiochemistry
- 1994
Two group I ribozymes, although differing in the identity of many of their active site nucleotides, nevertheless provide functionally similar active sites for sequence-specific RNA cleavage, and are concluded to be limited by the actual chemical cleavage step.
Deletion of nonconserved helices near the 3' end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity.
- Biology, ChemistryGenes & development
- 1988
Analysis of the cryptic site suggests that choice of the 3' splice site may not only depend on sequence but also on proximity to P9, a conserved helix at the 5' edge of the deletion point, which may be responsible for the reduced activity and accuracy of reactions with mutant precursors.
References
SHOWING 1-10 OF 43 REFERENCES
In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequence
- Biology, ChemistryCell
- 1981
The intervening sequence of the ribosomal RNA precursor is converted to a circular RNA in isolated nuclei of tetrahymena
- BiologyCell
- 1981
In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.
- BiologyNucleic acids research
- 1980
It is demonstrated that the enzyme activities necessary for the endonucleolytic cleavage as well as for the ligation of the transcript are associated with the isolated macronuclear rRNA genes of Tetrahymena thermophila.
Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
- 1979
R-loop hybridization and electron microscopy were used to map the RNA transcription products of the extrachromosomal rRNA genes of Tetrahymena thermophila, and preliminary evidence for a primary transcript that contains the intervening sequence was found.
The intervening sequence excised from the ribosomal RNA precursor of Tetrahymena contains a 5-terminal guanosine residue not encoded by the DNA.
- Biology, ChemistryNucleic acids research
- 1982
The splicing mechanisms are fundamentally different for yeast tRNA intervening sequences and Tetrahymena thermophila transcripts, leading to the conclusion that the splicing mechanism for these two classes of transcripts is fundamentally different.
The nucleotide sequence at the transcription termination site of the ribosomal RNA gene in Tetrahymena thermophila.
- Biology, ChemistryNucleic acids research
- 1982
The sequence of 415 nucleotides surrounding the transcription termination site for ribosomal RNA in Tetrahymena thermophila has been determined and a cluster of T's at the putative termination site, and several other T clusters are found further downstream.
The intervening sequence in the 26s rrna coding region of t. thermophila is transcribed within the largest stable precursor for rRNA
- BiologyCell
- 1979
Detailed transcription map of the extrachromosomal ribosomal RNA genes in Tetrahymena thermophila.
- BiologyJournal of molecular biology
- 1980
Sequence of a ribosomal RNA gene intron from Tetrahymena
- BiologyNature
- 1980
The sequence of a ribosomal RNA gene intron from Tetrahymena is reported in which intron–exon junctions differ from those analysed to date, suggesting different splicing activities are necessary for the processing of introns in mRN A and tRN A precursors.