Self-renewing human bone marrow mesenspheres promote hematopoietic stem cell expansion.

@article{Isern2013SelfrenewingHB,
  title={Self-renewing human bone marrow mesenspheres promote hematopoietic stem cell expansion.},
  author={Joan Isern and Beatriz Mart{\'i}n-Antonio and Roshanak Ghazanfari and Ana Mar{\'i}a Mart{\'i}n and Juan A L{\'o}pez and Raquel del Toro and Abel S{\'a}nchez-Aguilera and Lorena Arranz and Daniel Mart{\'i}n-P{\'e}rez and Mar{\'i}a Su{\'a}rez-Lled{\'o} and Pedro Mar{\'i}n and Melissa van Pel and Willem E. Fibbe and Jes{\'u}s V{\'a}zquez and Stefan Scheding and Alvaro Urbano-Ispizua and Sim{\'o}n M{\'e}ndez-Ferrer},
  journal={Cell reports},
  year={2013},
  volume={3 5},
  pages={
          1714-24
        }
}
Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and… CONTINUE READING

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