Self-directed and self-oriented immobilization of antibody by protein G-DNA conjugate.

@article{Jung2007SelfdirectedAS,
  title={Self-directed and self-oriented immobilization of antibody by protein G-DNA conjugate.},
  author={Yongwon Jung and Jeong Min Lee and Hyungil Jung and Bong Hyun Chung},
  journal={Analytical chemistry},
  year={2007},
  volume={79 17},
  pages={6534-41}
}
A versatile biolinker for efficient antibody immobilization was prepared by site-specific coupling of protein G to DNA oligonucleotide. This protein G-DNA conjugate ensures the controlled immobilization of an antibody to the intended area on the surface of bioassay chips or particles, while maintaining the activity and orientation of the bound antibody. Streptococcus protein G tagged with a cysteine residue at the N-terminus was chemically linked to amine-modified, single-stranded DNA. SPR… CONTINUE READING

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