Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection.

Abstract

We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes (FITC, Alexa647 and BODIPy-TR), mixed with pluronic F68 and linear polyethyleneimine (PEI), and emulsified by microfluidization. Prepared nanoemulsions (<200 nm) were readily taken up by both phagocytic and non-phagocytic cells in vitro after a short (approximately 3 h) co-incubation. Following cell administration in vivo, 19F MRI selectively visualizes cell migration. Exemplary in vivo MRI images are presented of T cells labeled with a dual-mode nanoemulsion in a BALB/c mouse. Fluorescence detection enables fluorescent microscopy and FACS analysis of labeled cells, as demonstrated in several immune cell types including Jurkat cells, primary T cells and dendritic cells. The intracellular fluorescence signal is directly proportional to the 19F NMR signal and can be used to calibrate cell loading in vitro.

DOI: 10.1021/ja077388j

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@article{Janjic2008SelfdeliveringNF, title={Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection.}, author={Jelena M. Janjic and Mangala Srinivas and Deepak K K Kadayakkara and Eric T. Ahrens}, journal={Journal of the American Chemical Society}, year={2008}, volume={130 9}, pages={2832-41} }