Self-cleaving transcripts of satellite DNA from the newt

@article{Epstein1987SelfcleavingTO,
  title={Self-cleaving transcripts of satellite DNA from the newt},
  author={Lloyd M. Epstein and Joseph G. Gall},
  journal={Cell},
  year={1987},
  volume={48},
  pages={535-543}
}
Self-cleaving viroid and newt RNAs may only be active as dimers
TLDR
More stable active structures that contain two self-cleavage sites are proposed for the plus and minus ASBV RNAs and the newt RNA5 and data supporting these models are presented.
The newt ribozyme is part of a riboprotein complex.
TLDR
It is shown that the newt ribozyme is in fact present as a riboprotein particle of about 12 S in the oocytes of Triturus and that an appropriate oligoribonucleotide substrate is specifically cleaved by the riboproteic activity in S-100 ovary extracts.
Newt satellite 2 transcripts self-cleave by using an extended hammerhead structure
TLDR
It is shown that cleavage by a single newt hammerhead requires additional satellite 2 sequences flanking both ends of the hammerhead domain, and a structural model of a truncated satellite 2 transcript which is capable of cleavage is presented.
Characterization of self-cleaving RNA sequences on the genome and antigenome of human hepatitis delta virus
TLDR
It was determined that the minimum length of contiguous sequence needed for self-cleavage of genomic RNA was 30 bases 5' and 74 bases 3' of the cleavage site, which was not compatible with the "hammerhead" structure hypothesized to be important in the self- Cleavage reactions of other RNAs.
Antigenomic RNA of human hepatitis delta virus can undergo self-cleavage
TLDR
In vitro the RNA complementary to the HDV genome, the antigenomic RNA, could undergo a self-cleavage that was not only more than 90% efficient but also occurred only at a single location.
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It is proposed that the stable cytoplasmic transcripts in the genome of the newt are encoded by a small subset of genomic satellite 2 sequences.
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Self-cleavage of both plus and minus RNA transcripts of the 247-residue avocado sunblotch viroid (ASBV), prepared from tandem dimeric cDNA clones, occurs specifically at two sites in each transcript
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