Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site

  title={Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active site},
  author={Anthony C. Forster and Robert H. Symons},
Characterization of self-cleaving RNA sequences on the genome and antigenome of human hepatitis delta virus
It was determined that the minimum length of contiguous sequence needed for self-cleavage of genomic RNA was 30 bases 5' and 74 bases 3' of the cleavage site, which was not compatible with the "hammerhead" structure hypothesized to be important in the self- Cleavage reactions of other RNAs.
RNA Self-Cleavage by the Hammerhead Structure
The in vitro RNA self-cleavage reactions that have been extensively characterized in viroid, virusoid and satellite RNAs, in hepatitis delta virus (HDV) RNA and in an RNA transcript of a repetitive DNA element in the genome of the newt are discovered.
Self-cleaving viroid and newt RNAs may only be active as dimers
More stable active structures that contain two self-cleavage sites are proposed for the plus and minus ASBV RNAs and the newt RNA5 and data supporting these models are presented.
A pseudoknot-like structure required for efficient self-cleavage of hepatitis delta virus RNA
It is proposed that the genomic self-cleaving sequence element4 and a corresponding sequence from the anti-genomic RNA could generate related secondary structures and evidence for two stems which form a tertiary interaction was obtained by site-specific mutagenesis of the antigenomic sequence.
Evidence that alternate foldings of the hepatitis delta RNA confer varying rates of self-cleavage.
It is proposed that this sequence alteration causes a refolding of the RNA, resulting in a "structural compensation" of the active core of the molecule, which could be a useful adaptation in viruses or in prebiotic RNAs.
Restriction in the cleavage activity of hammerhead ribozymes ensures ongoing evolution in prebiotic RNA world
Self-cleaving infectious RNAs found in many plant viruses and viroids can also cleave intrans and form hammerhead type secondary structure and the high electronegative nature of guanosine holds the key to its resistance to cleavage.
Antigenomic RNA of human hepatitis delta virus can undergo self-cleavage
In vitro the RNA complementary to the HDV genome, the antigenomic RNA, could undergo a self-cleavage that was not only more than 90% efficient but also occurred only at a single location.


Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sites
Non-enzymatic cleavage and ligation of RNAs complementary to a plant virus satellite RNA
The transcription in vitro of a circularly permuted, complementary DNA clone of STobRV RNA oriented so as to produce RNA that is complementary to the encapsidated, (+) polarity STob RV RNA is reported.
Satellite tobacco ringspot virus RNA: A subset of the RNA sequence is sufficient for autolytic processing.
It is shown here that transcripts of full-length and truncated DNA clones of the satellite RNA sequence also process in a nonenzymic reaction, and one such transcript was an RNA that has about one-fourth of theatellite RNA sequence, representing the 3'-terminal and 5-terminal portions of monomeric RNA joined in the junction that is cleaved in dimeric RNA.
Self-cleaving transcripts of satellite DNA from the newt
Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid.
Self-cleavage of both plus and minus RNA transcripts of the 247-residue avocado sunblotch viroid (ASBV), prepared from tandem dimeric cDNA clones, occurs specifically at two sites in each transcript
Autolytic Processing of Dimeric Plant Virus Satellite RNA
Evidence is presented for the in vitro autolytic processing of dimeric and trimeric forms of this satellite RNA of tobacco ringspot virus, which apparently is reversible to form dimeric RNA from monomeric RNA, and does not require an enzyme for its catalysis.
The Tetrahymena ribozyme acts like an RNA restriction endonuclease
A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
It is shown that single stranded RNA probes of a high specific activity are easy to prepare and can significantly increase the sensitivity of nucleic acid hybridization methods.