Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sites

@article{Forster1987SelfcleavageOP,
  title={Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sites},
  author={A. C. Forster and R. Symons},
  journal={Cell},
  year={1987},
  volume={49},
  pages={211-220}
}
Virusoids are circular single-stranded RNAs dependent on plant viruses for replication and encapsidation. Virusoid replication appears to involve longer-than-unit-length plus and minus RNAs, indicating that unit-length plus RNA is generated by specific cleavage reactions. Here, we synthesize plus and minus partial-length RNAs of the 324-nucleotide virusoid from lucerne transient streak virus in vitro. Both RNAs self-cleave at a unique site in the presence of magnesium ions to give 5' hydroxyl… Expand
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