Self assembly of NuMA: multiarm oligomers as structural units of a nuclear lattice.

@article{Harborth1999SelfAO,
  title={Self assembly of NuMA: multiarm oligomers as structural units of a nuclear lattice.},
  author={Jens Harborth and Jianghui Wang and C Gueth-Hallonet and Kathleen Weber and Mark Osborn},
  journal={The EMBO journal},
  year={1999},
  volume={18 6},
  pages={1689-700}
}
NuMA is a nuclear matrix protein in interphase and relocates to the spindle poles in mitotis. Different NuMA constructs, in which either N- or C-terminal domains were deleted, and the full-length construct were expressed in Escherichia coli, and the NuMA polypeptides were purified to homogeneity and allowed to assemble in vitro. Electron microscopy showed that NuMA can build multiarm oligomers by interaction of the C-terminal globular domains. Each arm of the oligomer corresponds to a NuMA… CONTINUE READING

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