Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address.

@article{Jongsma2006SelfassemblingPA,
  title={Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address.},
  author={Maarten A. Jongsma and Ralph H G M Litjens},
  journal={Proteomics},
  year={2006},
  volume={6 9},
  pages={2650-5}
}
The high-throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self-assembly concepts. DNA-directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O6-alkylguanine-DNA-alkyltransferase (SNAP-tag… CONTINUE READING

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