Self-Replicating RNA.

  title={Self-Replicating RNA.},
  author={Birke Andrea Tews and Gregor Meyers},
  journal={Methods in molecular biology},
Self-replicating RNA derived from the genomes of positive strand RNA viruses represents a powerful tool for both molecular studies on virus biology and approaches to novel safe and effective vaccines. The following chapter summarizes the principles how such RNAs can be established and used for design of vaccines. Due to the large variety of strategies needed to circumvent specific pitfalls in the design of such constructs the technical details of the experiments are not described here but can… CONTINUE READING

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