Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy

@article{Greenfield2009SelfOrganizationOT,
  title={Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy},
  author={D. L. Greenfield and Ann L. McEvoy and Hari Shroff and Gavin E. Crooks and Ned S. Wingreen and Eric Betzig and Jan T. Liphardt},
  journal={PLoS Biology},
  year={2009},
  volume={7}
}
Photoactivated localization microscopy analysis of chemotaxis receptors in bacteria suggests that the non-random organization of these proteins results from random self-assembly of clusters without direct cytoskeletal involvement or active transport. 
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References

SHOWING 1-10 OF 65 REFERENCES
Self-organized periodicity of protein clusters in growing bacteria.
TLDR
It is demonstrated within a lattice model that such spatial organization could arise spontaneously from a stochastic nucleation mechanism, which may explain the recent observation of periodic aggregates of misfolded proteins in E. coli.
Spatial Regulators for Bacterial Cell Division Self-Organize into Surface Waves in Vitro
TLDR
A reaction-diffusion model of the MinD and MinE dynamics is presented that accounts for the experimental observations and also captures the in vivo oscillations and formed planar surface waves on a flat membrane in vitro.
Spatial organization of the bacterial chemotaxis system.
Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy
TLDR
It is demonstrated that chemotaxis in E. coli requires the presence of chemoreceptor arrays and that the formation of these arrays requires the scaffolding interactions of the signaling molecules CheA and CheW.
High-density mapping of single-molecule trajectories with photoactivated localization microscopy
TLDR
This work created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables.
Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
TLDR
N nanoscale resolution in far-field fluorescence microscopy is demonstrated using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells by asynchronously recording the photon bursts of individual molecular switching cycles.
Positioning of chemosensory clusters in E. coli and its relation to cell division
TLDR
Fluorescence imaging is used to study cluster distribution and follow cluster dynamics during cell growth and shows that lateral clusters localise to specific periodic positions along the cell body, which mark future division sites and are involved in the localisation of the replication machinery.
Evolutionary Conservation of Methyl-Accepting Chemotaxis Protein Location in Bacteria andArchaea
TLDR
In elongated cells, the MCPs are located both at the poles and at regions along the length of the cells, suggesting that MCP location is evolutionarily conserved.
Electron Microscopic Analysis of Membrane Assemblies Formed by the Bacterial Chemotaxis Receptor Tsr
ABSTRACT The serine receptor (Tsr) from Escherichia coli is representative of a large family of transmembrane receptor proteins that mediate bacterial chemotaxis by influencing cell motility through…
Functional interactions between receptors in bacterial chemotaxis
TLDR
The results indicate that assemblies of bacterial chemoreceptors work in a highly cooperative manner, mimicking the behaviour of allosteric proteins, consistent with a number of models that assume long-range cooperative interactions between receptors as a general mechanism for signal integration and amplification.
...
...