Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers

  title={Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers},
  author={Jeffrey D. Hartgerink and Elia Beniash and Samuel I. Stupp},
  pages={1684 - 1688}
We have used the pH-induced self-assembly of a peptide-amphiphile to make a nanostructured fibrous scaffold reminiscent of extracellular matrix. The design of this peptide-amphiphile allows the nanofibers to be reversibly cross-linked to enhance or decrease their structural integrity. After cross-linking, the fibers are able to direct mineralization of hydroxyapatite to form a composite material in which the crystallographic c axes of hydroxyapatite are aligned with the long axes of the fibers… 

Peptide Self-Assembly into Hydrogels for Biomedical Applications Related to Hydroxyapatite

Amphiphilic peptides can be self-assembled by establishing physical cross-links involving hydrogen bonds and electrostatic interactions with divalent ions. The derived hydrogels have promising

Self-assembly of trifunctional tripeptides to form neural scaffolds.

The synthesis of a novel β-amino acid is described to allow for the synthesis ofA trifunctional β-tripeptide that remarkably maintains self-assembly and acts as a bioactive neuronal scaffold.

Modulating the gelation properties of self-assembling peptide amphiphiles.

It was found that the PAs could be combined to successfully control and stabilize the gelation properties, allowing for a mechanically tunable scaffold with increased durability, thereby improving the vast potential for cell encapsulation and other tissue engineering applications.

Slow-release RGD-peptide hydrogel monoliths.

It is proved that Fmoc-RGD monoliths can be used to release and encapsulate material, including model hydrophilic dyes and drug compounds, and provides the first insight into the correlation between the absorption and release kinetics of this new material.

Peptide-based fibrous biomaterials: Some things old, new and borrowed.

Design of Tissue-engineered Nanoscaffold Through Self-assembly of Peptide Amphiphile

The attachment, proliferation and osteogenic differentiation of MSCs were influenced by the self-assembled PA nanofibers as the cell scaffold and the values were significantly high compared with those in the static culture (2-D tissue culture plate).

Amphiphilic Histidine-Based Oligopeptides Exhibit pH-Reversible Fibril Formation

The design, simulation, synthesis, and reversible self-assembly of nanofibrils using polyhistidine-based oligopeptides creates the potential to produce well-defined fibrils for biotechnological and biomedical applications that are pH-responsive in a physiologically relevant range.

Enhanced Stability of Peptide Nanofibers Coated with a Conformal Layer of Polydopamine.

The surface of nanofibers formed by  β -sheet peptide self-assembly were coated by polydopamine (PDA) deposition, rendering the nanofiber dimensions and internal  p -stacking chirality impervious to changes in pH, temperature and physical processing by spin-coating onto a silicon wafer.

A comparison of peptide amphiphile nanofiber macromolecular assembly strategies

A new way of modeling supramolecular peptide nanofibers that better fits with the experimental data is presented and fibers which were modeled to be tilting the peptide amphiphile molecules and/or tilts the plane were found to be more stable and consistent with the experiments.

Mimicking bone nanostructure by combining block copolymer self-assembly and 1D crystal nucleation.

Using block copolymer-decorated polymer nanofibers to achieve biomineralized fibrils with precise control of both mineral crystal orientation and spatial distribution is reported.



Biomineralization: Chemical and Biochemical Perspectives

The functional forms of biominerals, R.J.P.Williams crystallochemical strategies in biomineralization, S.Mann carbonate calcification in algae - initiation and control, M.A.Borowitzka matrix-crystal

Electron microscopy in Biology: A practical approach

References and Notes

our experimentation could eventually be used to discredit our findings, should they happen not to agree with the original observations. It seems important that all experiments in the rapidly

Factors Controlling Long- and Short-Term Sequestration of Atmospheric CO2 in a Mid-latitude Forest

Legacies of prior disturbance and management, especially stand age and composition, controlled carbon uptake on the decadal time scale, implying that eastern forests could be managed for sequestration of carbon.

The arrangement of subunits agrees well with the following contacts identified by chemical cross-linking: Arp3 to p34, p21, and p20; Arp2 to p40 and p34 (long linker)

    For supplemental information, see Science Online

      Intermolecular and Sur face forces; 2nd ed.; Academic

      • London San Diego,
      • 1992

      We thank W. Kwiatkowski for help with the illustrations and computer systems, D. Davies of NIH for providing hospitality for

      • Beltzner for helpful suggestions on the text. Supported by NIH research grants GM-26132 and GM-26338 ( T.P.) and GM-56653 (S.C.), a Pioneer Foundation Fellowship (R.R.), an NRSA Fellowship

      The PA was characterized by 1 H nuclear magnetic resonance (NMR)

        Web figures 1 and 2 are available on Science Online at www