Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers

@article{Hartgerink2001SelfAssemblyAM,
  title={Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers},
  author={Jeffrey D. Hartgerink and Elia Beniash and Samuel I. Stupp},
  journal={Science},
  year={2001},
  volume={294},
  pages={1684 - 1688}
}
We have used the pH-induced self-assembly of a peptide-amphiphile to make a nanostructured fibrous scaffold reminiscent of extracellular matrix. The design of this peptide-amphiphile allows the nanofibers to be reversibly cross-linked to enhance or decrease their structural integrity. After cross-linking, the fibers are able to direct mineralization of hydroxyapatite to form a composite material in which the crystallographic c axes of hydroxyapatite are aligned with the long axes of the fibers… 

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The attachment, proliferation and osteogenic differentiation of MSCs were influenced by the self-assembled PA nanofibers as the cell scaffold and the values were significantly high compared with those in the static culture (2-D tissue culture plate).

Amphiphilic Histidine-Based Oligopeptides Exhibit pH-Reversible Fibril Formation

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Enhanced Stability of Peptide Nanofibers Coated with a Conformal Layer of Polydopamine.

The surface of nanofibers formed by  β -sheet peptide self-assembly were coated by polydopamine (PDA) deposition, rendering the nanofiber dimensions and internal  p -stacking chirality impervious to changes in pH, temperature and physical processing by spin-coating onto a silicon wafer.

A comparison of peptide amphiphile nanofiber macromolecular assembly strategies

A new way of modeling supramolecular peptide nanofibers that better fits with the experimental data is presented and fibers which were modeled to be tilting the peptide amphiphile molecules and/or tilts the plane were found to be more stable and consistent with the experiments.

Mimicking bone nanostructure by combining block copolymer self-assembly and 1D crystal nucleation.

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References and Notes

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The arrangement of subunits agrees well with the following contacts identified by chemical cross-linking: Arp3 to p34, p21, and p20; Arp2 to p40 and p34 (long linker)

    For supplemental information, see Science Online

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      We thank W. Kwiatkowski for help with the illustrations and computer systems, D. Davies of NIH for providing hospitality for

      • Beltzner for helpful suggestions on the text. Supported by NIH research grants GM-26132 and GM-26338 ( T.P.) and GM-56653 (S.C.), a Pioneer Foundation Fellowship (R.R.), an NRSA Fellowship

      The PA was characterized by 1 H nuclear magnetic resonance (NMR)

        Web figures 1 and 2 are available on Science Online at www