Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure.

@article{Wilds2002SeleniumassistedNA,
  title={Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure.},
  author={Christopher J Wilds and Rekha Pattanayek and Chongle Pan and Zdzislaw Wawrzak and Martin Egli},
  journal={Journal of the American Chemical Society},
  year={2002},
  volume={124 50},
  pages={14910-6}
}
The combination of synchrotron radiation and a variety of atoms or ions (either covalently attached to the biomolecule prior to crystallization or soaked into crystals) that serve as anomalous scatterers constitutes a powerful tool in the X-ray crystallographer's repertoire of structure determination techniques. Phosphoroselenoates in which one of the nonbridging phosphate oxygens in the backbone is replaced by selenium offer a simplified means for introducing an anomalous scatterer into… CONTINUE READING