Selenate reductase activity in Escherichia coli requires Isc iron-sulfur cluster biosynthesis genes.

  title={Selenate reductase activity in Escherichia coli requires Isc iron-sulfur cluster biosynthesis genes.},
  author={Nathan W. Yee and Jessica K. Choi and Abigail W Porter and Sean Carey and Ines Rauschenbach and Arye Harel},
  journal={FEMS microbiology letters},
  volume={361 2},
The selenate reductase in Escherichia coli is a multi-subunit enzyme predicted to bind Fe-S clusters. In this study, we examined the iron-sulfur cluster biosynthesis genes that are required for selenate reductase activity. Mutants devoid of either the iscU or hscB gene in the Isc iron-sulfur cluster biosynthesis pathway lost the ability to reduce selenate. Genetic complementation by the wild-type sequences restored selenate reductase activity. The results indicate the Isc biosynthetic system… 

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