Selectivity of substrate binding and ionization of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase.

@article{Luanloet2015SelectivityOS,
  title={Selectivity of substrate binding and ionization of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase.},
  author={Thikumporn Luanloet and Jeerus Sucharitakul and Pimchai Chaiyen},
  journal={The FEBS journal},
  year={2015},
  volume={282 16},
  pages={3107-25}
}
2-Methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase (EC 1.14.12.4) from Pseudomonas sp. MA-1 is a flavin-dependent monooxygenase that catalyzes a hydroxylation and aromatic ring cleavage reaction. The functional roles of two residues, Tyr223 and Tyr82, located ~ 5 Å away from MHPC, were characterized using site-directed mutagenesis, along with… CONTINUE READING