Selectively-infective phage (SIP): a mechanistic dissection of a novel in vivo selection for protein-ligand interactions.

@article{Krebber1997SelectivelyinfectiveP,
  title={Selectively-infective phage (SIP): a mechanistic dissection of a novel in vivo selection for protein-ligand interactions.},
  author={Claus M. Krebber and Stefania Spada and Dominique Desplancq and Anke Krebber and Lei Ge and Andreas Pluckthun},
  journal={Journal of molecular biology},
  year={1997},
  volume={268 3},
  pages={
          607-18
        }
}
Selectively-infective phage (SIP) is a novel methodology for the in vivo selection of interacting protein-ligand pairs. It consists of two components, (1) a phage particle made non-infective by replacing its N-terminal domains of geneIII protein (gIIIp) with a ligand-binding protein, and (2) an "adapter" molecule in which the ligand is linked to those N-terminal domains of gIIIp which are missing from the phage particle. Infectivity is restored when the displayed protein binds to the ligand and… CONTINUE READING

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