Selectively dispersed isotope labeling for protein structure determination by magic angle spinning NMR

@article{Eddy2013SelectivelyDI,
  title={Selectively dispersed isotope labeling for protein structure determination by magic angle spinning NMR},
  author={Matthew T Eddy and Marina Belenky and Astrid C. Sivertsen and Robert G Griffin and Judith Herzfeld},
  journal={Journal of Biomolecular NMR},
  year={2013},
  volume={57},
  pages={129-139}
}
The power of nuclear magnetic resonance spectroscopy derives from its site-specific access to chemical, structural and dynamic information. However, the corresponding multiplicity of interactions can be difficult to tease apart. Complimentary approaches involve spectral editing on the one hand and selective isotope substitution on the other. Here we present a new “redox” approach to the latter: acetate is chosen as the sole carbon source for the extreme oxidation numbers of its two carbons… CONTINUE READING
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