Selective transfer of individual human chromosomes to recipient cells.

  title={Selective transfer of individual human chromosomes to recipient cells.},
  author={P J Saxon and Eri S. Srivatsan and G V Leipzig and J H Sameshima and Eric John Stanbridge},
  journal={Molecular and cellular biology},
  volume={5 1},
Two hypoxanthine phosphoribosyltransferase-deficient human cell lines, D98/AH-2 and HT1080-6TG, were stably transfected with pSV2 gpt, a plasmid containing the selectable marker Escherichia coli xanthine-guanine phosphoribosyl transferase (Eco gpt). Hypoxanthine-aminopterin-thymidine-resistant transformants arose with a frequency of ca. 10(-6) and contained mostly single, but occasionally multiple, copies of the plasmid sequences. These transformants actively express the Eco gpt marker. Single… CONTINUE READING


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