Selective sequestration of X4 isolates by human genital epithelial cells: Implication for virus tropism selection process during sexual transmission of HIV

@article{Berlier2005SelectiveSO,
  title={Selective sequestration of X4 isolates by human genital epithelial cells: Implication for virus tropism selection process during sexual transmission of HIV},
  author={Willy Berlier and Thomas Bourlet and Philip Lawrence and Hind Hamzeh and Claude Lambert and Christian Genin and Bernard Verrier and Marie-Caroline Dieu-Nosjean and Bruno Pozzetto and Olivier Del{\'e}zay},
  journal={Journal of Medical Virology},
  year={2005},
  volume={77}
}
X4 and R5 HIV strains are present in the semen of men infected with HIV but R5 isolates are transmitted preferentially. The role of human epithelial cells in this selection is addressed. Three human cervical cell lines—CaSki, SiHa, and HEC1A—and normal human vaginal cells from HIV‐negative donors were characterized for HIV receptor expression and incubated with X4 and R5 laboratory‐adapted strains or primary isolates. The infection was assessed by detection of intracellular HIV DNA. The three… 
View on Wiley
ncbi.nlm.nih.gov
50 Citations
Highly Influential Citations
1
Background Citations
17
Methods Citations
1
Results Citations
3

50 Citations

Selective transmigration of monocyte-associated HIV-1 across a human cervical monolayer and its modulation by seminal plasma

The preferential transmigration of HIV R5 strains associated with monocytes across the endocervical monolayer may explain the predominant transmission of the R5 strain after sexual intercourse.

Construction and tropism characterisation of recombinant viruses exhibiting HIV-1 env gene from seminal strains.

R5- and X4-HIV-1 use differentially the endometrial epithelial cells HEC-1A to ensure their own spread: implication for mechanisms of sexual transmission.

Oral epithelial cells and their interactions with HIV-1.

It is clear that, whilst not primary targets for infection and virus replication, epithelial cells play an important role in the infection cycle and improving the understanding of their interactions with HIV could potentially provide key insights necessary to develop effective preventative therapies.

The spreading of HIV-1 infection in the human organism is caused by fractalkine trafficking of the infected lymphocytes—a review, hypothesis and implications for treatment

The present review attempts to define the role of chemokine receptors like CCR5 and CXCR4, and especially fractalkine receptor CX3CR1 in the trafficking of lymphocytes in healthy individuals and HIV-1/AIDS patients.

A novel VSV/HIV pseudotype approach for the study of HIV microbicides without requirement for level 3 biocontainment

It is shown that soluble Gb3 analogs inhibit in vitro infection of cervical and vaginal-derived cell lines by both intact HIV and the VSV/HIV recombinant virus.

Medroxyprogesterone Acetate Regulates HIV-1 Uptake and Transcytosis but Not Replication in Primary Genital Epithelial Cells, Resulting in Enhanced T-Cell Infection.

This is the first report that HIV uptake by GECs increased significantly in the presence of the hormonal contraceptive medroxyprogesterone acetate (MPA) and progesterone and that uptake occurred primarily via endocytosis.

Seminal plasma promotes the attraction of Langerhans cells via the secretion of CCL20 by vaginal epithelial cells: involvement in the sexual transmission of HIV.

The results demonstrate that epithelial cells respond to factors present in semen by secreting CCL20, leading to the enhancement of LCp recruitment, arguing in favour of the implication of epithelium cells in the heterosexual transmission of HIV.

HIV Infection in gastric epithelial cells.

The present data suggest that the gastric epithelial cells are natural targets of HIV infection, and HIV infection in epithelial Cells contributes to HIV-induced gastric mucosal inflammation.

Potential mechanisms for increased HIV-1 transmission across the endocervical epithelium during C. trachomatis infection.

In vitro and in vivo results suggests several mechanisms for increased transmission of HIV-1 across the endocervical epithelial cell line of C. trachomatis-infected women, including a dramatic increase in the number of CD4+, CXCR4 and/or CCR5 positive T cell targets in the endOCervix.

References

SHOWING 1-10 OF 29 REFERENCES

Human genital epithelial cells capture cell-free human immunodeficiency virus type 1 and transmit the virus to CD4+ Cells: implications for mechanisms of sexual transmission.

By use of an ectocervical epithelium-derived cell line, it is found that the cells are capable of sequestering large amounts of HIV particles but are refractory to cell-free viral infection.

Dendritic Cell–T-Cell Interactions Support Coreceptor-Independent Human Immunodeficiency Virus Type 1 Transmission in the Human Genital Tract

It is indicated that HIV-1 can exploit T-cell–DC synergism in the human genital tract to overcome potential coreceptor restrictions on DC and postentry blocks of viral replication in unactivated T cells.

Human Immunodeficiency Virus Type 1 Infection and Replication in Normal Human Oral Keratinocytes

Examining the possibility that normal human oral keratinocytes (NHOKs) might be directly infected by HIV or might convey infectious HIV virions to adjacent leukocytes suggests a potential role of oral epithelial cells in the transmission of HIV infection.

Functional HIV CXCR4 coreceptor on human epithelial Langerhans cells and infection by HIV strain X4

It is demonstrated that CXCR4 is present on the surface of Langerhans cells freshly isolated from human skin epidermis and that this expression is functional.

Macrophage-tropic variants initiate human immunodeficiency virus type 1 infection after sexual, parenteral, and vertical transmission.

Genotypical and phenotypical characteristics of virus populations, present in sexual, parenteral, or vertical donor-recipient pairs, were studied and homogeneous virus populations of macrophage-tropic, non-syncytium-inducing, HIV-1 variants were present prior to seroconversion.

Human immunodeficiency virus type 1 infection of cells and tissues from the upper and lower human female reproductive tract

It is demonstrated that HIV-1 can infect cells and tissues from different sites within the female reproductive tract and suggest that multiple cell types, including epithelial cells, may be targets for the initial infection by HIV- 1.

Essential roles for dendritic cells in the pathogenesis and potential treatment of HIV disease.

Topical microbicides that interfere with HIV infection of Langerhans cells represent an attractive strategy for blocking sexual transmission of virus and dendritic cells have been targeted both ex vivo and in vivo to initiate and enhance HIV-specific immunity.

Parameters of Human Immunodeficiency Virus Infection of Human Cervical Tissue and Inhibition by Vaginal Virucides

This is the first demonstration that virucidal agents designed for topical vaginal use block HIV infection of genital tissue, and has major implications for world health, as they will provide women with a mechanism of personal and covert protection from HIV infection.

Infection of vaginal and colonic epithelial cells by the human immunodeficiency virus type 1 is neutralized by antibodies raised against conserved epitopes in the envelope glycoprotein gp120.

It is demonstrated that vaginal epithelial cells can be infected by HIV type 1 (HIV-1) via a mechanism similar to that described for neuroglial cells and, more recently, for colorectal epithel cells, involving initial interaction of the HIV-1 envelope glycoprotein gp120 with a cell-surface glycosphingolipid (sulfated lactosylceramide).

Genetic characterization of human immunodeficiency virus type 1 in blood and genital secretions: evidence for viral compartmentalization and selection during sexual transmission

In every pair studied, the transmitted variant(s) represents only a minor population in the semen of the corresponding transmitter, thereby providing evidence that HIV-1 selection indeed occurs during sexual transmission.