Selective sequestration of X4 isolates by human genital epithelial cells: Implication for virus tropism selection process during sexual transmission of HIV

  title={Selective sequestration of X4 isolates by human genital epithelial cells: Implication for virus tropism selection process during sexual transmission of HIV},
  author={Willy Berlier and Thomas Bourlet and Philip Lawrence and Hind Hamzeh and Claude Lambert and Christian Genin and Bernard Verrier and Marie-Caroline Dieu-Nosjean and Bruno Pozzetto and Olivier Del{\'e}zay},
  journal={Journal of Medical Virology},
X4 and R5 HIV strains are present in the semen of men infected with HIV but R5 isolates are transmitted preferentially. The role of human epithelial cells in this selection is addressed. Three human cervical cell lines—CaSki, SiHa, and HEC1A—and normal human vaginal cells from HIV‐negative donors were characterized for HIV receptor expression and incubated with X4 and R5 laboratory‐adapted strains or primary isolates. The infection was assessed by detection of intracellular HIV DNA. The three… 

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