Selective removal of undifferentiated human embryonic stem cells using magnetic activated cell sorting followed by a cytotoxic antibody.

@article{Schriebl2012SelectiveRO,
  title={Selective removal of undifferentiated human embryonic stem cells using magnetic activated cell sorting followed by a cytotoxic antibody.},
  author={Kornelia Schriebl and Gernalia Satianegara and Austin Hwang and Heng Liang Tan and Wey Jia Fong and Henry He Yang and Alois Jungbauer and A. Choo},
  journal={Tissue engineering. Part A},
  year={2012},
  volume={18 9-10},
  pages={899-909}
}
One of the most pertinent concerns of using differentiated cells derived from human embryonic stem cells (hESC) is the presence of residual undifferentiated hESC, because they carry a risk of teratoma formation. A new cell-cell separation approach that eliminates teratoma-forming hESC in order to ensure safer cell therapy was developed. By combining antibodies (IgMs or IgGs) for the selective removal of undifferentiated hESC using magnetic activated cell sorting (MACS) followed by selective… CONTINUE READING