Selective reactivity of rhodanese sulfhydryl groups with 5,5'-dithio-bis(2-nitrobenzoic acid).


The reactivity of the sulfhydryl groups of sulfur-containing and sulfur-free rhodanese (thiosulfate : cyanide sulfurtransferase, EC with 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) has been investigated. Only 0.6 sulfhydryl group of the sulfur-containing enzyme reacts with DTNB. After removal of sulfur from persulfide groups a further 0.6… (More)


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