Selective protection against the cytotoxicity of methotrexate and methotrexate-poly(lysine) by thiamine pyrophosphate, heparin and leucovorin.

@article{Shen1981SelectivePA,
  title={Selective protection against the cytotoxicity of methotrexate and methotrexate-poly(lysine) by thiamine pyrophosphate, heparin and leucovorin.},
  author={Wei Shen and Hugues J.-P. Ryser},
  journal={Life sciences},
  year={1981},
  volume={28 11},
  pages={
          1209-14
        }
}
  • W. Shen, H. Ryser
  • Published 16 March 1981
  • Biology, Chemistry, Materials Science
  • Life sciences
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Drug-Poly(Lysine) Conjugates: Their Potential for Chemotherapy and for the Study of Endocytosis
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Targeting, internalization, and cytotoxicity of methotrexate-monoclonal anti-stage-specific embryonic antigen-1 antibody conjugates in cultured F-9 teratocarcinoma cells.
TLDR
Results indicate that the MTX antibody conjugate binds specifically to F-9 cells, and is internalized and intracellularly degraded to release a small molecular active drug.
Drug Delivery with Protein and Peptide Carriers
TLDR
The mechanism by which the drug is taken up into a cellular target may be altered for the drug-carrier complex and, in the case of an antibody carrier, tissue specificity may also be added to the properties of the drug.

References

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Poly (L-lysine) and poly (D-lysine) conjugates of methotrexate: different inhibitory effect on drug resistant cells.
TLDR
Conjugation of methotrexate to poly(L-lysine) markedly increases its cellular uptake and offers a new way to overcome drug resistance related to deficient transport and to give rise in the cell to a pharmacologically active breakdown product.
Photoinactivation of the methotrexate transport system of L1210 cells by 8-azidoadenosin 5'-monophosphate.
TLDR
It is shown that complete photoinactivation of methotrexate transport could not be obtained from a single exposure to 8-azido-AMP, but it could be achieved by the repetitive illumination of cells in a fresh medium and the phosphate and folate/adenine transport systems of L1210 cells are not affected by irradiation in the presence of 7-azidoadenosine-AMP.