Selective propagation of retinal pericytes in mixed microvascular cell cultures using L-leucine-methyl ester.

@article{Lee1998SelectivePO,
  title={Selective propagation of retinal pericytes in mixed microvascular cell cultures using L-leucine-methyl ester.},
  author={Chonho Lee and Wayne F Patton and N Chung-Welch and Eddie T. Chiang and K H Spofford and David Shepro},
  journal={BioTechniques},
  year={1998},
  volume={25 3},
  pages={
          482-8, 490-2, 494
        }
}
Endothelial cell (EC) propagation has been simplified by developing cell-specific selection criteria. Methods commonly used for selectively isolating EC include: (i) differential sieving of disaggregated tissue, (ii) differential plating of cells on extracellular matrices, (iii) lectin affinity isolation of cell populations and (iv) fluorescence-activated cell sorting of cells labeled with a carbocyanine dye of acetylated low-density lipoprotein (DiI-Ac-LDL). Few criteria for selectively… CONTINUE READING
2 Citations
26 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 26 references

Hydrogen peroxide - induced filamin translocation in an in vitro model of reoxygenation injury ; Inhibition by iron chela - tors and superoxide radical scavengers

  • L. Hastie, W. Patton, H. Hechtman, D. Shepro
  • Free Radic . Biol . Med .
  • 1997

Bradykinin - induced filamin translocation in endothelial cells ; regulation by calcium , protein kinases and protein phosphatases

  • Q. Wang, W. Patton, E. Chiang
  • J . Cell . Biochem .
  • 1996

Biologist ’ s perspective on analytical imaging systems as applied to protein gel electrophoresis

  • M. Taubman, P. Tempst
  • J . Chromatogr . A
  • 1995

Human microvascular endothelial cells : isolation , culture and characterization

  • P. Hewett
  • In Vitro Cell . Dev . Biol .
  • 1993

Similar Papers

Loading similar papers…