Selective plane illumination microscopy techniques in developmental biology.

@article{Huisken2009SelectivePI,
  title={Selective plane illumination microscopy techniques in developmental biology.},
  author={Jan Huisken and Didier Y. R. Stainier},
  journal={Development},
  year={2009},
  volume={136 12},
  pages={1963-75}
}
Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited… CONTINUE READING
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