Selective phylogenetic analysis targeting 16S rRNA genes of hyperthermophilic archaea in the deep-subsurface hot biosphere.

@article{Kimura2007SelectivePA,
  title={Selective phylogenetic analysis targeting 16S rRNA genes of hyperthermophilic archaea in the deep-subsurface hot biosphere.},
  author={Hiroyuki Kimura and Jun-ichiro Ishibashi and Harue Masuda and Kenji Kato and Satoshi Hanada},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 7},
  pages={2110-7}
}
International drilling projects for the study of microbial communities in the deep-subsurface hot biosphere have been expanded. Core samples obtained by deep drilling are commonly contaminated with mesophilic microorganisms in the drilling fluid, making it difficult to examine the microbial community by 16S rRNA gene clone library analysis. To eliminate mesophilic organism contamination, we previously developed a new method (selective phylogenetic analysis [SePA]) based on the strong… CONTINUE READING

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