Selective photochemical treatment of oestrogen receptor in a Xenopus liver extract destroys hormone binding and transcriptional activation but not DNA binding.

@article{Cridland1990SelectivePT,
  title={Selective photochemical treatment of oestrogen receptor in a Xenopus liver extract destroys hormone binding and transcriptional activation but not DNA binding.},
  author={N A Cridland and Christopher V E Wright and Edward A. McKenzie and John Knowland},
  journal={The EMBO journal},
  year={1990},
  volume={9 6},
  pages={1859-66}
}
Photochemical excitation of a simple derivative of oestradiol using light in the UV-A range totally, permanently and selectively inactivates the oestrogen receptor protein present in a Xenopus liver extract without affecting its overall size. Inactivation of the binding site proceeds to completion with simple, first-order kinetics. Inactivation is prevented… CONTINUE READING