Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

@article{Alt1978SelectiveMO,
  title={Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.},
  author={Frederick W Alt and Rodney E. Kellems and Joseph R. Bertino and Robert T. Schimke},
  journal={The Journal of biological chemistry},
  year={1978},
  volume={253 5},
  pages={1357-70}
}
The rate of dihydrofolate reductase synthesis in the AT3000 line of methotrexate-resistant murine Sarcoma 180 cells is approximately 200to 250-fold greater than that of the sensitive, parental line. We have purified cDNA sequences complementary to dihydrofolate reductase mRNA and subsequently used this probe to quantitate dihydrofolate reductase mRNA and gene copies in each of these lines. Analysis of the association kinetics of the purified cDNA with DNA from sensitive and resistant cells… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 161 extracted citations

References

Publications referenced by this paper.

Cold Spring Harbor Symp

K. D. Tartof
Quant . BioZ • 1973

Similar Papers

Loading similar papers…