Selective modification of aspartic acid-101 in lysozyme by carbodiimide reaction.

@article{Yamada1981SelectiveMO,
  title={Selective modification of aspartic acid-101 in lysozyme by carbodiimide reaction.},
  author={H. Yamada and Taiji Imoto and K. Fujita and K Okazaki and Masato Motomura},
  journal={Biochemistry},
  year={1981},
  volume={20 17},
  pages={
          4836-42
        }
}
A general procedure which selectively introduced a nucleophilic group at a particular location in the active site of lysozyme has been developed. The coupling of hen egg white lysozyme with amine nucleophiles by 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride (EDC) was studied at pH 5 and room temperature. In the presence of an amine nucleophile, such as ethanolamine, ethylenediamine, methylamine, or 4(5)-(aminomethyl)imidazole, the carboxyl side chain of aspartic acid-101 in… Expand
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The chemical evidence for the enzymic activity of lysozyme will be discussed in detail by other speakers at this meeting, but in order to describe our crystallographic studies of the interactionsExpand
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