Selective inhibition of genetic transcription in sea urchin embryos. Incorporation of 5-bromodeoxyuridine into low molecular weight nuclear DNA.

@article{Kotzin1972SelectiveIO,
  title={Selective inhibition of genetic transcription in sea urchin embryos. Incorporation of 5-bromodeoxyuridine into low molecular weight nuclear DNA.},
  author={Brian L. Kotzin and Robert Baker},
  journal={The Journal of cell biology},
  year={1972},
  volume={55 1},
  pages={74-81}
}
Experimental evidence suggests that exposure of sea urchin gastrulae to 5-bromodeoxyuridine (BUdR), an analog of thymidine, causes a reduction in the rate of synthesis of some RNA species usually transcribed at this stage of development. In pulse-chase experiments, (3)H (in gastrula stage 8-15S nuclear DNA labeled with BUdR-(3)H) could not be chased, with unlabeled BUdR, into 20-60S size-range DNA; in similar experiments in which gastrulae were pulsed with thymidine-(3)H and then chased with… CONTINUE READING
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