Selective growth of normal adult human urothelial cells in serum-free medium

@article{Kirk1985SelectiveGO,
  title={Selective growth of normal adult human urothelial cells in serum-free medium},
  author={David Kirk and Susumu Kagawa and Gudrun Vener and Karthik S. Narayan and Yasushi Ohnuki and Lawrence W. Jones},
  journal={In Vitro Cellular & Developmental Biology},
  year={1985},
  volume={21},
  pages={165-171}
}
A serum-free medium (HMRI-2) has been developed for the outgrowth and subculture of epithelial cells from normal adult human ureter and bladder. Medium HMRI-2 consists of Ham’s MCDB 152 with double the amounts of the essential amino acids in Stock 1, low Ca2+ (0.06 mM) and is supplemented with epithelial growth factor, 5 ng/ml; transferrin, 5 μg/ml; insulin… CONTINUE READING