Selective formation of mannosyl-l-arabitol lipid by Pseudozyma tsukubaensis JCM16987

  title={Selective formation of mannosyl-l-arabitol lipid by Pseudozyma tsukubaensis JCM16987},
  author={Tomotake Morita and Tokuma Fukuoka and Aya Kosaka and Tomohiro Imura and Hideki Sakai and Masahiko Abe and Dai Kitamoto},
  journal={Applied Microbiology and Biotechnology},
To develop a structural homolog of mannosylerythritol lipids (MELs), Pseudozyma tsukubaensis JCM16987 (known to be a specific producer of the diastereomer type of mono-acetylated MEL (MEL-B)) was cultivated in medium containing 4 % (w/v) olive oil as the primary carbon source and 4 % l-arabitol as the supplemental sugar alcohol. Based on thin-layer chromatography (TLC), the glycolipid extract showed two major spots corresponding to MEL-B and an unknown glycolipid (GL1). Based on high… Expand
A novel approach for the production and purification of mannosylerythritol lipids (MEL) by Pseudozyma tsukubaensis using cassava wastewater as substrate
Abstract P. tsukubaensis is a yeast-like microorganism that synthesized the biosurfactant mannosylerythritol lipids-B (MEL-B). Production cost can be one of the drawbacks of biosurfactantsExpand
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The present two glycolipids were identified as mannosylribitol lipid (MRL) and mannosylerythritol lipids (MAL), respectively, which facilitate the application of biosurfactants as new functional materials. Expand
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Of the strains tested, P. parantarctica gave the best yield of MELs (30 g L(-1); thus, MEL production may be an important taxonomic index for the Pseudozyma yeasts. Expand
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The present strain of P. parantarctica provided high efficiency in MEL production at elevated temperatures compared to conventional MEL producers, and would thus be highly advantageous for the commercial production of the promising biosurfactants. Expand
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In the course of MEL production from soybean oil by P. antarctica and P. rugulosa, some new extracellular glycolipids (more hydrophobic than the previously reported di-acylated MELs) were found in the culture medium. Expand