Selective esterase-ester pair for targeting small molecules with cellular specificity.

@article{Tian2012SelectiveEP,
  title={Selective esterase-ester pair for targeting small molecules with cellular specificity.},
  author={Lin Tian and Yunlei Yang and Laura M. Wysocki and Alma Arnold and Amy Yichen Hu and Balaji Ravichandran and Scott Sternson and Loren L. Looger and Luke D Lavis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 13},
  pages={4756-61}
}
Small molecules are important tools to measure and modulate intracellular signaling pathways. A longstanding limitation for using chemical compounds in complex tissues has been the inability to target bioactive small molecules to a specific cell class. Here, we describe a generalizable esterase-ester pair capable of targeted delivery of small molecules to living cells and tissue with cellular specificity. We used fluorogenic molecules to rapidly identify a small ester masking motif that is… CONTINUE READING