Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets.

@article{He2005SelectiveEO,
  title={Selective encapsulation of single cells and subcellular organelles into picoliter- and femtoliter-volume droplets.},
  author={Mingyan He and John Scott Edgar and Gavin D. M. Jeffries and Robert Meyer Lorenz and Johanna Shelby and Daniel T Chiu},
  journal={Analytical chemistry},
  year={2005},
  volume={77 6},
  pages={1539-44}
}
This paper describes a method, which combines optical trapping and microfluidic-based droplet generation, for selectively and controllably encapsulating a single target cell or subcellular structure, such as a mitochondrion, into a picoliter- or femtoliter-volume aqueous droplet that is surrounded by an immiscible phase. Once the selected cell or organelle is encased within the droplet, it is stably confined in the droplet and cannot be removed. We demonstrate in droplet the rapid laser… CONTINUE READING

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