Selective determination of cysteines through precolumn double-labeling and liquid chromatography followed by detection of intramolecular FRET.

Abstract

In this paper we introduce a novel approach for highly selective and sensitive analysis of cysteines (glutathione, cysteine, and homocysteine). This method is based on the detection of intramolecular fluorescence resonance energy transfer (FRET) in a liquid chromatography (LC) system after double-labeling of the amino and sulfhydryl groups of the cysteines… (More)
DOI: 10.1007/s00216-010-4461-6

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