Selective ceramide binding to protein kinase C-alpha and -delta isoenzymes in renal mesangial cells.

@article{Huwiler1998SelectiveCB,
  title={Selective ceramide binding to protein kinase C-alpha and -delta isoenzymes in renal mesangial cells.},
  author={Andrea Huwiler and Doriano Fabbro and Josef M. Pfeilschifter},
  journal={Biochemistry},
  year={1998},
  volume={37 41},
  pages={
          14556-62
        }
}
Ceramide is an important lipid second messenger produced by sphingolipid metabolism in cells exposed to a limited number of agonists and in turn triggers several cell responses in a protein kinase C (PKC)-dependent manner. Stimulation of mesangial cells with a radioiodinated photoaffinity labeling analogue of ceramide, (N-[3-[[[2-(125I)iodo-4-[3-(trifluoromethyl)-3H-diazirin-3-yl]benz yl] oxy]carbonyl]propanoyl]-D-erythro-sphingosine) ([125I]-TID-ceramide), defines PKC-alpha and PKC-delta as… 
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