Selective assay for herpes simplex viruses expressing thymidine kinase.

@article{CampionePiccardo1979SelectiveAF,
  title={Selective assay for herpes simplex viruses expressing thymidine kinase.},
  author={J. Campione-Piccardo and WilliamE. Rawls and Silvia Bacchetti},
  journal={Journal of virology},
  year={1979},
  volume={31 2},
  pages={
          281-7
        }
}
A technique for selecting herpes simplex viruses expressing the viral thymidine kinase (TK+) from a population of predominantly TK- viruses was developed. This was accomplished by infecting TK- cells and incubating the cultures under a liquid overlay medium containing methotrexate. Since the TK- cells survive in this medium for only a limited period of time, it was necessary to add fresh uninfected TK- cells 48 h after infection. The technique allowed the detection and quantitation of the TK… CONTINUE READING

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