Selective and sensitive colorimetric detection of stringent alarmone ppGpp with Fenton-like reagent.

@article{Zheng2014SelectiveAS,
  title={Selective and sensitive colorimetric detection of stringent alarmone ppGpp with Fenton-like reagent.},
  author={Lin Ling Zheng and Cheng Zhi Huang},
  journal={The Analyst},
  year={2014},
  volume={139 23},
  pages={
          6284-9
        }
}
Stringent alarmone, namely, guanosine 3'-diphosphate-5'-diphosphate (ppGpp), is a global regulator that plays a critical role in the survival, growth, metabolism, and many other vital processes of microorganisms. Because of its structural similarity to normal nucleotides, it is also a challenge for the selective and sensitive detection of ppGpp nowadays. Herein, we developed a colorimetric method for the selective detection of ppGpp by inhibiting the redox reaction between Fenton-like reagent… Expand
Ratiometric fluorescence detection of stringent ppGpp using Eu-MoS2 QDs test paper
Abstract Guanosine 3′-diphosphate-5′-diphophate (ppGpp) as a stringent alarmone plays an important role in survival, growth, metabolism processes of bacteria and plants. However, it is still a greatExpand
Colorimetric Logic Gate for Pyrophosphate and Pyrophosphatase via Regulating the Catalytic Capability of Horseradish Peroxidase.
TLDR
The proposed analytical system has been verified to have potential to be utilized for enzyme inhibitor screening and diagnosis of PPase-related diseases and to have high sensitivity and selectivity, cost-effectiveness, and simplification. Expand
Colorimetric determination of the activity of acetylcholinesterase and its inhibitors by exploiting the iodide-catalyzed oxidation of 3,3′,5,5′-tetramethylbenzidine by hydrogen peroxide
AbstractWe describe a sensitive and selective colorimetric method for the determination of the activity of the enzyme acetylcholinesterase (AChE) and its inhibitors. Detection is based on the factExpand
Live-Cell Imaging of (p)ppGpp with RNA-based Fluorescent Sensors
TLDR
Unprecedented information on cell-to-cell variation and cellular dynamics of (p)ppGpp levels can now be obtained under different nutritional conditions and it is predicted that these RNA-based sensors will be broadly adapted to study bacterial stringent response. Expand
A simple and rapid colorimetric sensor for sulfide anion detection based on redox reaction of ABTS with Au (III)
A cost-effective and simple colorimetric sensor based on redox reaction of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with Au (III) ion, has been fabricated for the determinationExpand
Polyphosphate Functions In Vivo as an Iron Chelator and Fenton Reaction Inhibitor
TLDR
PolyP, a universally conserved biomolecule, plays a hitherto unrecognized role as an iron chelator and an inhibitor of the Fenton reaction, which shows that polyP provides a crucial iron reservoir for metalloproteins under nonstress conditions and effectively chelates free iron during iron stress. Expand
Polyphosphate Functions In Vivo as Iron Chelator and Fenton Inhibitor
TLDR
It is demonstrated that polyP, a universally conserved biomolecule, plays a hitherto unrecognized role as an iron chelator and an inhibitor of the Fenton reaction, and provides a crucial iron reservoir under non-stress conditions. Expand

References

SHOWING 1-10 OF 48 REFERENCES
Selective fluorometric detection of pyrophosphate and stringent alarmone with copper(II)-2,6-bis(2-benzimidazolyl)pyridine complex.
TLDR
A selective fluorometric detection method for PPi with a copper(II) complex of 2,6-bis(2-benzimidazolyl)pyridine (bbimp) and then applied it to the detection of bacterial alarmone ppGpp. Expand
Highly selective detection of bacterial alarmone ppGpp with an off-on fluorescent probe of copper-mediated silver nanoclusters.
TLDR
This work achieves highly selective detection of bacterial alarmone in a broad range concentration of 2-200 μM, but also improves the understanding of the specific recognitions among DNA-Ag NCs, Cu(2+), and ppGpp. Expand
A radioimmunoassay for (p)ppGpp and its application to Streptomyces hygroscopicus
TLDR
The very sensitive radioimmunoassay allowed determination of very low amounts: 1–50 pmoles (p)ppGpp per assay tube and the application of the RIA to S. hygroscopicus revealed a basal level in exponentially growing mycelium of about 2 pmoles. Expand
Novel findings on the copper catalysed oxidation of cysteine
SummaryThe oxidation of cysteine (RSH) has been studied by using O2, ferricytochrome c (Cyt c) and nitro blue tetrazolium (NBT) as electron acceptors. The addition of 200μM CuII to a solution of 2mMExpand
The Fenton reagents.
TLDR
The question whether free .OH radicals are being formed or not via the Fenton reaction depends on the relative rates of the decomposition reactions of the metal-peroxide complex and that of its reaction with organic substrates. Expand
Metabolic initiation of differentiation and secondary metabolism by Streptomyces griseus: significance of the stringent response (ppGpp) and GTP content in relation to A factor
  • K. Ochi
  • Medicine, Biology
  • Journal of bacteriology
  • 1987
TLDR
It was concluded that morphological differentiation of S. griseus results from a decrease in the pool of GTP, whereas physiological differentiationresults from a more direct function of the rel gene product (ppGpp). Expand
Colorimetric and fluorescent sensing of pyrophosphate in 100% aqueous solution by a system comprised of rhodamine B compound and Al3+ complex.
TLDR
The ensemble showed highly sensitive and selective fluorescent and colorimetric response to pyrophosphate among the anions in 100% aqueous solutions and no interference of the potent biological competitors including ATP, ADP, and phosphate. Expand
Application of cysteine monolayers for electrochemical determination of sub-ppb copper(II)
Electrodes with the functionality of amino acids can be easily prepared by taking advantage of strong sulfur−gold interactions and immersing gold electrodes into cysteine solutions. Such electrodesExpand
(p)ppGpp and drug resistance
TLDR
In recent years, emerging and reemerging pathogens resistant to nearly all available antibiotics are on the rise, thus it is imperative to develop new drugs to treat infections and to facilitate the discovery and development of novel antibiotics. Expand
Fenton-like Chemistry in Water: Oxidation Catalysis by Fe(III) and H2O2
The formation of active intermediates from the Fenton-like reagent (a mixture of iron(III) ions and hydrogen peroxide) in aqueous solution has been investigated using static DFT calculations andExpand
...
1
2
3
4
5
...