Selective analysis of histamine in food by means of solid-phase extraction cleanup and chromatographic separation.

@article{Oguri2007SelectiveAO,
  title={Selective analysis of histamine in food by means of solid-phase extraction cleanup and chromatographic separation.},
  author={Shigeyuki Oguri and Mayo Enami and Naoko Soga},
  journal={Journal of chromatography. A},
  year={2007},
  volume={1139 1},
  pages={70-4}
}
A simple, practical technique is presented for the selective determination and measurement of histamine (HA) levels in fermented food. The method involved a solid-phase extraction cleanup using a Sep-Pak Plus C-18 cartridge and ion-paired reversed-phase high-performance liquid chromatographic (IP-RP-HPLC) separation, followed by detection of HA at its UV absorbance wavelength of 220nm. After evaporating a methanolic extract from the food sample, the resulting residue was reconstituted with 0.2M… CONTINUE READING

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