Selective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs.

@article{Lavery1997SelectiveAV,
  title={Selective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs.},
  author={Daniel J Lavery and Luis Lopez-Molina and Fabienne Fleury-Olela and Ueli Schibler},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 13},
  pages={6831-6}
}
We present a novel subtractive enrichment protocol for the identification of differentially expressed mRNA species. This procedure, called SABRE (selective amplification via biotin- and restriction-mediated enrichment), uses selective streptavidin-biotin affinity and restriction enzyme site reconstitution to enrich for cDNA species more abundant in one population than in another. Analysis of liver cDNA from a mouse strain expressing the neomycin resistance gene demonstrated that this procedure… CONTINUE READING
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