Selective Tryptophan Oxidation of Monoclonal Antibodies: Oxidative Stress and Modeling Prediction.

@article{Pavon2019SelectiveTO,
  title={Selective Tryptophan Oxidation of Monoclonal Antibodies: Oxidative Stress and Modeling Prediction.},
  author={Jorge Alexander Pavon and Li Guang Xiao and Xiaojuan Li and Ji-wen Zhao and Danielle L Aldredge and Eugene Dank and Alex Fridman and Yan-Hui Liu},
  journal={Analytical chemistry},
  year={2019},
  volume={91 3},
  pages={
          2192-2200
        }
}
Oxidation of tryptophan not only generates heterogeneity of a therapeutic monoclonal antibody (mAb) but also can be a potential critical quality attribute (CQA) of the product. In this study, mAbs A-C of IgG1 and IgG4 (immunoglobulin G, IgG) isotypes with oxidized tryptophan (Trp) residues were selectively generated by incubating the mAbs with 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) in formulations containing l-methionine. The site-specific oxidation of tryptophan residues were… CONTINUE READING