Selective Toxins and Analogs Produced by Helminthosporium sacchari: Production, Characterization, and Biological Activity.

  title={Selective Toxins and Analogs Produced by Helminthosporium sacchari: Production, Characterization, and Biological Activity.},
  author={R. Livingston and R. Scheffer},
  journal={Plant physiology},
  volume={76 1},
Helminthosporium sacchari toxin and several lower molecular weight, nontoxic analogs were isolated from culture filtrates. Three isomers of the toxin (A, B, and C), each with four galactose units, were separated by high performance liquid chromatography. Isomer C had the highest and isomer A had the lowest toxicity to H. sacchari-susceptible sugarcane; resistant clones were not affected. Each toxin isomer was partially digested with a beta-galactofuranosidase and the resulting analogs (seven… Expand
Interconversions of aglycone and host-selective toxin from Helminthosporium sacchari
The results suggest a metabolic route (aglycone → metabolite Y, → HS toxin → metabolites X and Y are lower molecular weight homologues of the toxin) for the biosynthesis of HS toxin. Expand
Cellular target of helminthosporium sacchari Toxin in sugarcane tissue
Spindles from sugarcane tissue were challenged using labeled Helminthosporium (Bipolaris) sacchari toxin to determine the general effect on different organelles and structures, and a more efficient toxin labeling than previously reported was obtained. Expand
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Isolation and characterization of host-selective toxin from Helminthosporium sacchari.
Comparative data show that the toxin examined in this study is the same as the toxin described by Steiner and Strobel, and the previously proposed structure is incorrect. Expand
Conversion of Helminthosporium sacchari Toxin to Toxoids by beta-Galactofuranosidase from Helminthosporium.
An enzyme with beta-galactofuranosidase activity was found in small amounts in the cultures of H. sacchari; the enzyme converted toxin to the toxoids in vitro, and more work is needed to determine the significance of enzyme production by these fungi. Expand
Biological Activity of the Isomeric Forms of Helminthosporium sacchari Toxin and of Homologs Produced in Culture.
The effect of Helminthosporium sacchari (HS) toxin isomers and related, pathogen-produced compounds on dark CO(2) fixation in HS-susceptible sugar cane leaf slices was investigated and should prove a useful tool for understanding the mode of action of HS toxin. Expand
Toxic and protective effects of analogues of Helminthosporium sacchari toxin on sugarcane tissues
Toxoids protect sugarcane tissues from toxin, as shown by use of the assay based on toxin-induced loss of electrolytes, and suggest that toxoids act as competitive inhibitors of HS toxin. Expand
Structure of the host-specific pathotoxins produced by Phyllosticta maydis
Abstract Linear polyketol structures of two host-specific pathotoxins from Phyllosticta maydis (PM-toxin B and PM-toxin C) were elucidated by MS fragmentation patterns of the toxins and theirExpand
Rapid chromatographic technique for preparative separations with moderate resolution
(11) Potassium ferricyanide has previously been used to convert w'c-1,2-dicarboxylate groups to double bonds. See, for example, L. F. Fieser and M. J. Haddadln, J. Am. Chem. Soc., 86, 2392 (1964).Expand