Selective Photostimulation of Genetically ChARGed Neurons

@article{Zemelman2002SelectivePO,
  title={Selective Photostimulation of Genetically ChARGed Neurons},
  author={Boris V. Zemelman and Georgia A. Lee and Minna Ng and Gero Miesenb{\"o}ck},
  journal={Neuron},
  year={2002},
  volume={33},
  pages={15-22}
}
To permit direct functional analyses of neural circuits, we have developed a method for stimulating groups of genetically designated neurons optically. Coexpression of the Drosophila photoreceptor genes encoding arrestin-2, rhodopsin (formed by liganding opsin with retinal), and the alpha subunit of the cognate heterotrimeric G protein--an explosive combination we term "chARGe"--sensitizes generalist vertebrate neurons to light. Illumination of a mixed population of neurons elicits action… Expand
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