Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression

Abstract

Structural characterization of membrane proteins and other large proteins with NMR relies increasingly on perdeuteration combined with incorporation of specifically protonated amino acid moieties, such as methyl groups of isoleucines, valines, or leucines. The resulting proton dilution reduces dipolar broadening producing sharper resonance lines… (More)
DOI: 10.1021/ja504791j

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