Selection of valid reference genes for expression studies of hepatic cell lines under IFN-α treatment.

@article{Hashemi2012SelectionOV,
  title={Selection of valid reference genes for expression studies of hepatic cell lines under IFN-α treatment.},
  author={Atieh Hashemi and Farzin Roohvand and M Ghahremani},
  journal={Biochemical and biophysical research communications},
  year={2012},
  volume={426 4},
  pages={649-53}
}
The proper selection of reference genes to normalize the quantitative real-time PCR (RT-qPCR) results under particular experimental conditions is crucial for validation of the gene quantification data. Herein, using SYBR green RT-qPCR, five reference genes (GAPDH, ACTB, HMBS, HPRT-1 and TBP) were evaluated to determine the most stable reference genes in hepatic cell lines (Huh-7 and HepG(2)) under IFN-α treatment conditions. Analyses by geNorm program ranked GAPDH and HPRT-1 in Huh-7 and that… CONTINUE READING