Selection of transgenic flax plants is facilitated by spectinomycin

@article{BretagneSagnard2005SelectionOT,
  title={Selection of transgenic flax plants is facilitated by spectinomycin},
  author={B{\'e}r{\'e}nice Bretagne-Sagnard and Yves Chupeau},
  journal={Transgenic Research},
  year={2005},
  volume={5},
  pages={131-137}
}
Regeneration of transformed flax shoots after inoculation withAgrobacterium tumefaciens carrying a binary vector with either a neomycin phosphotransferase (nptII) gene and a β-glucuronidase (GUS) reporter gene or a spectinomycin resistance gene was examined. Hypocotyls from 4-day-old seedlings were inoculated with either of the twoA. tumefaciens strains. Selection and regeneration were achieved on a medium containing 0.1 μM thidiazuron, 0.01 μM napthalene acetic acid, 100 mgl−1 kanamycin… CONTINUE READING
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