Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.)

@inproceedings{Campos2015SelectionOS,
  title={Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.)},
  author={Maria Doroteia Campos and Ant{\'o}nio Miguel Frederico and Thomas Nothnagel and Birgit Arnholdt-Schmitt and H{\'e}lia G Cardoso},
  year={2015}
}
Reverse transcription quantitative real time polymerase chain reaction (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression studies. Appropriate application of RT-qPCR requires accurate normalization through the use of suitable reference genes. This study aimed selecting robust and reliable reference genes which are constitutively and equally expressed for accurate RT-qPCR normalization analysis in two different experimental systems with carrot. A systematic… CONTINUE READING

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