Selection of single-stranded DNA molecules that bind and inhibit human thrombin

  title={Selection of single-stranded DNA molecules that bind and inhibit human thrombin},
  author={Louis C. Bock and Linda C. Griffin and John A. Latham and Eric H. Vermaas and John Jay Toole},
APTAMERS1 are double-stranded DNA or single-stranded RNA molecules that bind specific molecular targets. Large randomly generated populations can be enriched in aptamers by in vitro selection and polymerase chain reaction1–11. But so far single-stranded DNA has not been investigated for aptamer properties, nor has a target protein been considered that does not interact physiologically with nucleic acid. Here we describe the isolation of single-stranded DNA aptamers to the protease thrombin of… CONTINUE READING
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