Selection of single-chain variable fragment antibodies to black currant reversion associated virus from a synthetic phage display library.

@article{Susi1998SelectionOS,
  title={Selection of single-chain variable fragment antibodies to black currant reversion associated virus from a synthetic phage display library.},
  author={Petri Susi and Angelika Ziegler and Lesley Torrance},
  journal={Phytopathology},
  year={1998},
  volume={88 3},
  pages={230-3}
}
ABSTRACT Single-chain variable fragment (scFv) antibodies that bind to black currant reversion associated virus (BRAV) were obtained from a synthetic phage display antibody gene library without recourse to animal immunizations. Several different BRAV-specific phage scFv were obtained quickly, after only three rounds of selection against immobilized virus antigen. The phage scFv gave enzyme-linked immunosorbent assay (ELISA) absorbance values that were greater than seven times the control… CONTINUE READING