Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus

Abstract

Amphioxus is a closest living proxy to the ancestor of cephalochordates with vertebrates, and key animal for novel understanding in the evolutionary origin of vertebrate body plan, genome, tissues and immune system. Reliable analyses using quantitative real-time PCR (qRT-PCR) for answering these scientific questions is heavily dependent on reliable… (More)
DOI: 10.1038/srep37549

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