Selection of reliable reference genes for gene expression studies in Trichoderma afroharzianum LTR-2 under oxalic acid stress.

@article{Lyu2017SelectionOR,
  title={Selection of reliable reference genes for gene expression studies in Trichoderma afroharzianum LTR-2 under oxalic acid stress.},
  author={Yuping Lyu and Xiaoqing Wu and H. Ren and F. Zhou and Hongzi Zhou and Xinjian Zhang and Hetong Yang},
  journal={Journal of microbiological methods},
  year={2017},
  volume={141},
  pages={
          28-31
        }
}
An appropriate reference gene is required to get reliable results from gene expression analysis by quantitative real-time reverse transcription PCR (qRT-PCR). In order to identify stable and reliable reference genes in Trichoderma afroharzianum under oxalic acid (OA) stress, six commonly used housekeeping genes, i.e., elongation factor 1, ubiquitin, ubiquitin-conjugating enzyme, glyceraldehyde-3-phosphate dehydrogenase, α-tubulin, actin, from the effective biocontrol isolate T. afroharzianum… Expand
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