Selection of reliable reference genes for gene expression studies in Trichoderma afroharzianum LTR-2 under oxalic acid stress.
@article{Lyu2017SelectionOR, title={Selection of reliable reference genes for gene expression studies in Trichoderma afroharzianum LTR-2 under oxalic acid stress.}, author={Yuping Lyu and Xiaoqing Wu and H. Ren and F. Zhou and Hongzi Zhou and Xinjian Zhang and Hetong Yang}, journal={Journal of microbiological methods}, year={2017}, volume={141}, pages={ 28-31 } }
An appropriate reference gene is required to get reliable results from gene expression analysis by quantitative real-time reverse transcription PCR (qRT-PCR). In order to identify stable and reliable reference genes in Trichoderma afroharzianum under oxalic acid (OA) stress, six commonly used housekeeping genes, i.e., elongation factor 1, ubiquitin, ubiquitin-conjugating enzyme, glyceraldehyde-3-phosphate dehydrogenase, α-tubulin, actin, from the effective biocontrol isolate T. afroharzianum… Expand
Topics from this paper
9 Citations
Selection of reliable reference genes for gene expression studies in Botrytis cinerea.
- Biology, Medicine
- Journal of microbiological methods
- 2017
- 13
Validation of reference genes for quantitative gene expression analysis in Ralstonia pseudosolanacearum CQPS-1 under environment stress.
- Biology, Medicine
- Journal of microbiological methods
- 2018
- 4
Selection of reliable reference genes for RT-qPCR during methyl jasmonate, salicylic acid and hydrogen peroxide treatments in Ganoderma lucidum
- Chemistry, Medicine
- World journal of microbiology & biotechnology
- 2018
- 7
Identification of suitable reference genes for real-time qPCR in homocysteine-treated human umbilical vein endothelial cells
- Medicine, Biology
- PloS one
- 2018
- 2
- PDF
The effect of abiotic and biotic stress on the salicylic acid biosynthetic pathway from mandelonitrile in peach
- Biology
- 2017
- Highly Influenced
- PDF
YARS as an oncogenic protein that promotes gastric cancer progression through activating PI3K-Akt signaling
- Biology, Medicine
- Journal of Cancer Research and Clinical Oncology
- 2020
- 4
- PDF
Evolutionary convergence or homology? Comparative cytogenomics of Caesalpinia group species (Leguminosae) reveals diversification in the pericentromeric heterochromatic composition
- Biology, Medicine
- Planta
- 2019
- 5
Running title: Salicylic acid biosynthesis link to cyanogenesis
- 2017
References
SHOWING 1-10 OF 28 REFERENCES
Selection of reliable reference genes for gene expression studies in Clonostachys rosea 67-1 under sclerotial induction.
- Medicine, Biology
- Journal of microbiological methods
- 2015
- 13
Reference gene selection for normalization of RT-qPCR gene expression data from Actinidia deliciosa leaves infected with Pseudomonas syringae pv. actinidiae
- Biology, Medicine
- Scientific reports
- 2015
- 60
- PDF
Validation of Potential Reference Genes for qPCR in Maize across Abiotic Stresses, Hormone Treatments, and Tissue Types
- Biology, Medicine
- PloS one
- 2014
- 66
- PDF
Validation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots
- Biology, Medicine
- Scientific Reports
- 2017
- 15
- PDF
Identification of a Novel Reference Gene for Apple Transcriptional Profiling under Postharvest Conditions
- Biology, Medicine
- PloS one
- 2015
- 17
- PDF
Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)
- Biology, Medicine
- BMC Research Notes
- 2014
- 36
Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR
- Biology, Medicine
- BMC Molecular Biology
- 2006
- 918
- PDF
Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines
- Biology, Medicine
- PloS one
- 2013
- 153
- PDF
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations
- Biology, Medicine
- Biotechnology Letters
- 2004
- 3,446
- PDF
Antibiosis functions during interactions of Trichoderma afroharzianum and Trichoderma gamsii with plant pathogenic Rhizoctonia and Pythium
- Biology, Medicine
- Functional & Integrative Genomics
- 2015
- 11